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Endoplasmic Reticulum Associated Degradation of Spinocerebellar Ataxia-Related CD10 Cysteine Mutant Kanuka, Mai Ouchi, Fuka Kato, Nagisa Katsuki, Riko Ito, Saori Miura, Kohta Hikida, Masaki Tamura, Taku open access © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). spinocerebellar ataxia CD10 disulfide bond endoplasmic reticulum-associated degradation (ERAD) ER quality control Spinocerebellar ataxia (SCA) is one of the most severe neurodegenerative diseases and is often associated with misfolded protein aggregates derived from the genetic mutation of related genes. Recently, mutations in CD10 such as C143Y have been identified as SCA type 43. CD10, also known as neprilysin or neuroendopeptidase, digests functional neuropeptides, such as amyloid beta, in the extracellular region. In this study, we explored the cellular behavior of CD10 C143Y to gain an insight into the functional relationship of the mutation and SCA pathology. We found that wild-type CD10 is expressed on the plasma membrane and exhibits endopeptidase activity in a cultured cell line. CD10 C143Y, however, forms a disulfide bond-mediated oligomer that does not appear by the wild-type CD10. Furthermore, the CD10 C143Y mutant was retained in the endoplasmic reticulum (ER) by the molecular chaperone BiP and was degraded through the ER-associated degradation (ERAD) process, in which representative ERAD factors including EDEM1, SEL1L, and Hrd1 participate in the degradation. Suppression of CD10 C143Y ERAD recovers intracellular transport but not enzymatic activity. Our results indicate that the C143Y mutation in CD10 negatively a ects protein maturation and results in ER retention and following ERAD. These findings provide beneficial insight into SCA type 43 pathology. MDPI 2020 eng journal article VoR http://hdl.handle.net/10295/00005833 https://air.repo.nii.ac.jp/records/5516 https://doi.org/10.3390/ijms21124237 International Journal of Molecular Sciences 21 12 4237 https://air.repo.nii.ac.jp/record/5516/files/riA_2021_9.pdf application/pdf 3.3 MB 2021-11-25