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RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS
http://hdl.handle.net/10295/3139
http://hdl.handle.net/10295/3139b078cbb1-651f-4523-a6d7-60be80e0eabb
名前 / ファイル | ライセンス | アクション |
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akitai42-3_4(147).pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-08-01 | |||||
タイトル | ||||||
タイトル | RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IDH-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rapid immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glioma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | intraoperative diagnosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA sequencing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
HIROSHIMA, Yuko
× HIROSHIMA, Yuko× NANJO, Hiroshi× SASAJIMA, Toshio× SHIMIZU, Hiroaki× MINAMIYA, Yoshihiro× YOSHIOKA, Toshiaki× ODA, Masaya× KUDO-ASABE, Yukitsugu× TSUDA, Masumi× TANINO, Mishie× TANAKA, Shinya× AKAGAMI, Yoichi× GOTO, Akiteru |
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内容記述(抄録) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Immunohistochemistry (IHC) plays a major role in the histopathological diagnosis of central nervous system (CNS) tumors. We developed a rapid immunohistochemistry (R-IHC) method using an alternating current electric field, which enables the completion of IHC in 20 min. We performed intraoperative R-IHC on 16 glioma patients (9 with grade I-III gliomas and 7 with glioblastomas) using the anti-mutant isocitrate dehydrogenase 1 (IDH1) monoclonal antibody (IDH1- R132H ; H09), and the results were validated on permanent formalin-fixed paraffin-embedded samples of the same patients by standard IHC. DNA sequencing of the IDH1 gene was also performed for the 16 patients. Of 6 gliomas, 5 were shown to be positive by both R-IHC and standard IHC, and IDH1 mutation was confirmed by DNA sequencing. According to the results of R-IHC compared with both standard IHC results and DNA sequencing, the sensitivity of IDH1 R-IHC was 83% (5/6) ; specificity, 70% (7/10) ; positive predictive value, 63% (5/8) ; negative predictive value, 88% (7/8) ; and accuracy, 75% (12/16). Thus, R-IHC using the anti-mutant IDH1 monoclonal antibody has potential applications in the intraoperative identification of IDH1 mutations in gliomas. |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
書誌情報 |
秋田医学 巻 42, 号 3/4, p. 147-156, 発行日 2016-03-31 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03866106 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00009294 | |||||
出版者 | ||||||
出版者 | 秋田医学会 |