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INTRAOPERATIVE DETECTION OF LYMPH NODE MICROMETASTASIS USING A RAPID IMMUNOHISTOCHEMICAL STAINING METHOD IN NON-SMALL CELL LUNG CANCER
http://hdl.handle.net/10295/2156
http://hdl.handle.net/10295/215652fdf3fc-329b-4c2e-b9f4-9ebdf35c7dcb
名前 / ファイル | ライセンス | アクション |
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akitai39(73).pdf (936.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-05-29 | |||||
タイトル | ||||||
タイトル | INTRAOPERATIVE DETECTION OF LYMPH NODE MICROMETASTASIS USING A RAPID IMMUNOHISTOCHEMICAL STAINING METHOD IN NON-SMALL CELL LUNG CANCER | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | non-small cell lung cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lymph node metastasis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lymph node micrometastasis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunohistochemical staining | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kawaharada, Yasushi
× Kawaharada, Yasushi× Minamiya, Yoshihiro× Nanjo, Hirosi× Saito, Hajime× Konno, Hayato× Kagaya, Masam× Akagami, Yo-ichi× Motoyama, Satoru× Ogawa, Jun-ichi |
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内容記述(抄録) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nodal micrometastasis in non-small cell lung cancer (NSCLC) is associated with a poorer survival rate than node-negative disease. Furthermore, lymph node micrometastasis often cannot be detected using conventional hematoxylin and eosin staining of frozen sections ; detection requires additional time-consuming immunohistochemical (IHC) analysis of paraffin-embedded tissue. We previously developed and reported a device that enables us to complete IHC analyses within 11 minutes. In the present study, we used this rapid-IHC protocol with an anti-cytokeratin antibody and analyzed 205 mediastinal lymph nodes dissected during surgery for NSCLC. Although we modified the original rapid-IHC procedure to block endogenous peroxidase activity, which could potentially cause misdiagnosis, the staining was still completed within 19 min. On the basis of conventional histological examination, 7 lymph nodes from 3 patients were deemed positive for micrometastasis. By contrast using hematoxylin and eosin staining, 13 lymph nodes from 7 patients were diagnosed positive on the basis of cytokeratin-detection using routine-IHC, and the same 13 nodes were diagnosed positive on the basis of rapid-IHC. That is, all nodes deemed positive with the routine-IHC procedure were also positive with the rapid-IHC procedure. Assuming the results of the routine-IHC are correct, the sensitivity, specificity and accuracy of rapid-IHC are 100%, 100% and 100%, respectively. These findings demonstrate the utility of our rapid-IHC analysis for intraoperative diagnosis of micrometastasis. However, our findings are limited by the fact that we tested the method using a single antibody at a single institute. Further investigation in multicenter studies will be needed to confirm the utility of this method. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
書誌情報 |
秋田医学 巻 39, 号 2, p. 73-79, 発行日 2013-02-28 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03866106 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00009294 | |||||
出版者 | ||||||
出版者 | 秋田医学会 |